Laboratory Testing Directory

Laboratoy Testing Directory

Blood Sample Requirements: Blood Samples are accepted from 7:00 AM Monday till 2:00 PM Friday.
Federal regulations require all clinical blood specimens (tubes, containers) be labeled with the patient's full name (spelled correctly), birth date and sample date/time. If these requirements are not met, the lab must delay or refuse testing until compliance is met.


For pediatric sample volume contact the Clinical Laboratory.


A specimen may be rejected for any of the following reasons:
• Incorrect or compromised specimen (hemolysis, clotted specimens)
• Insufficient volume for analysis
• Unlabeled or improperly labeled specimen
• Specimen that has been sent in incorrect collection tube(s)
• Incomplete or incorrect testing requisition

Histocompatibility Testing

ABO Typing

Description: Determination of ABO blood group

Methodology: Serology

Test Code(s): ABO

Billing Code(s) w/ Fees: 86900 - $75

Days Performed: Monday - Friday, 0630-1630

Turn Around Time: 1 Day

STAT Turn Around Time: 1 Hour


Collection Requirements

Specimen: Whole Blood

Collection Container: (1) Lavender Top (EDTA). Also Acceptable: (1) Yellow Top (ACD-A)

Collection Volume: 6 mL


Specimen Processing Requirements

Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.

Test Limitations: Grossly hemolyzed samples will be rejected.

Donor Specific Antibody Identification Class I and II

Description: Test is used for the detection and identification of antibodies directed to donor-specific HLA class I and II antigens by single-antigen bead-based flow cytometry.

Methodology: Luminex Microbead Array

Test Code(s): PTXSAB1Fee, PTXSAB2Fee

Billing Code(s) w/ Fee(s): 86832 - $500, 86833 - $500

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days

STAT Turn Around Time: 24 Hours


Collection Requirements

Specimen: Whole Blood 

Collection Container: (1) Red Top

Collection Volume: 6-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

The laboratory will report mean fluorescence intensity (MFI) of beads bearing HLA antigen specific to the broad antigens mismatched to the transplant organ donor. Information may be useful in tracking and trending sensitization (or desensitization) to donor HLA antigens.

Test Limitations: Donor HLA typing required for interpretation.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Flow Crossmatch-Auto w/ Pronase

Description: Detection of the presence of autoantibodies (HLA) in a solid organ recipient.

Methodology: Flow Cytometry

Test Code(s): X-Auto T-Cell, X-Auto B-Cell

Billing Code(s) w/ Fee(s): T-Cell 86825 - $500, B-Cell 86825 - $500

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days


Collection Requirements

Specimen: Blood 

Collection Container: (3) ACD Tubes (Yellow Top), (1) Red Top

Collection Volume: 5-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

The laboratory will report crossmatch results "positive," "weak positive," or "negative" based on the change in fluorescent signal (median channel shift (MCS)). Any WEAK POSITIVE or POSITIVE result is directly approved by the Medical Director before reporting.

Test Limitations: A proper interpretation of crossmatch testing requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and/or interfering medications, substances (i.e., Rituximab Therapy) is provided to the laboratory PRIOR to testing any serum.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Flow Crossmatch - Living Donor w/ Pronase

Description: Evaluate the compatibility between potential transplant recipient and living donor.

Methodology: Flow Cytometry

Test Code(s): X-LD T-Cell, X-LD B-Cell

Billing Code(s) w/ Fee(s): T-cell 86825 - $500, B-cell 86825 - $500

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days

STAT Turn Around Time: 4-6 Hours


Collection Requirements

Specimen: Blood 

Collection Container: Donor - (3) ACD Tubes (Yellow Top), Patient - (1) Red Top

Collection Volume: 6-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

The laboratory will report crossmatch results “positive,” “weak positive,” or “negative,” based on the change in fluorescent signal (median channel shift (MCS)). Results should be interpretated in conjunction with Luminex antibody testing and clinical risk factors. Positive flow crossmatch may indicate increased risk of antibody mediated organ rejection. Any WEAK POSITIVE or POSITIVE result is directly approved by the Medical Director before reporting.

Test Limitations: A proper interpretation of crossmatch testing requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., Rituximab Therapy) is provided to the laboratory PRIOR to testing any serum.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

HLA Antibody Testing Class I

Description: Single Antigen Antibody specificities to HLA Class I antigens.

Methodology: Luminex Microbead Array

Test Code(s): SAB1

Billing Code(s) w/ Fee(s): 86832-$470

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days

STAT Turn Around Time: 6 Hours


Collection Requirements

Specimen: Whole Blood 

Collection Container: (1) Red Top

Collection Volume: 5-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

Results will list class I (HLA-A, -B, or -C locus) antibodies detected and the calculated percent reactive antibody (cPRA).

Test Limitations: A proper interpretation of antibodies requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., IVIG) is provided to the laboratory PRIOR to testing any serum.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

HLA Antibody Testing Class II

Description: Single Antigen Antibody specificities to HLA Class II antigens.

Methodology: Luminex Microbead Array

Test Code(s): SAB2

Billing Code(s) w/ Fee(s): 86833 - $470

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days

STAT Turn Around Time: 6 Hours


Collection Requirements

Specimen: Whole Blood 

Collection Container: (1) Red Top

Collection Volume: 5-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

Results will list class II (HLA-DR, -DQ, or -DP locus) antibodies detected and the calculated percent reactive antibody (cPRA).

Test Limitations: A proper interpretation of antibodies requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., IVIG) is provided to the laboratory PRIOR to testing any serum.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

HLA Typing Class I and II

Description: DNA based assay for HLA-A, B, C, DR, DR345 and DQB (Low resolution).

Methodology: Reverse SSO detected by Luminex xMAP

Test Code(s): T-HLA CL1+2

Billing Code(s) w/ Fee(s): 81370 - $1400

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 3-7 Days

STAT Turn Around Time: 6 Hours


Collection Requirements

Specimen: Whole Blood 

Collection Container: (2) Yellow Top (ACD-A), Also Acceptable: (2) Lavender Top (EDTA)

Collection Volume: 5-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

An HLA typing report will be provided.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

HLA Typing Class II Extended

Description: DNA based assay for HLA-DQA, DPA, DPB (Low Resolution)

Methodology: Reverse SSO detected by Luminex xMAP

Test Code(s): T-HLA CL2 Ext

Billing Code(s) w/ Fee(s): DQA 81376 - $250, DPA 81376 - $250, DPB 81376 - $250

Days Performed: Monday-Friday, 0630-1630

Turn Around Time: 2-5 Days

STAT Turn Around Time: 6 Hours


Collection Requirements

Specimen: Whole Blood 

Collection Container: (2) Yellow Top (ACD-A), Also Acceptable: (2) Lavender Top (EDTA)

Collection Volume: 5-10 mL


Specimen Processing Requirements

Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.


Interpretation

An HLA typing report will be provided.

Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director. 

This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).

STAT

Description: Reduced reporting time, MUST contact the laboratory to schedule

Test Code(s): Z-STAT Fee

Billing Code(s) w/ Fee(s): 86849 - $200

Days Performed: Monday-Friday, 0630-1630

STAT Turn Around Time: 6 Hours

STAT After Hours

Description: Reduced reporting time, MUST contact the laboratory to schedule

Test Code(s): Z-After Hours

Billing Code(s) w/ Fee(s): 86849 - $400

Days Performed: Monday-Friday after 1630, includes weekends

STAT Turn Around Time: 6 Hours

Surgical Pathology

Coming soon!